Regulatory

Part:BBa_K1973013:Design

Designed by: Laura Claret Fernndez   Group: iGEM16_UPO-Sevilla   (2016-10-09)


Pm (1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It did have to be site-directed mutagenized in position 65 in order to remove a PstI restriction site.


Source

It was obtained by PCR from a plamid that had its sequence.

References